LOCUS       pLV-[hsa-miR-181b-1]        9164 bp    DNA     circular     20-APR-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7085..7945
                     /label=Amp(R)
     promoter        8146..8686
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5690..5924
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6499..6954)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5996..6126
                     /label=SV40 pA
     misc_feature    6127..6340
                     /label=SV40 ori
     misc_feature    5011..5607
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3629
                     /label=intron
     CDS             3676..5001
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3419
                     /label=hsa-mir-181b-1 precursor
BASE COUNT     2180 a      2374 c      2467 g      2143 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatccc cgttgattgt 
     3121 accctatggc taaccatcat ctactccatg gtgctcagaa ttcgctgaag acaggaaacc 
     3181 aaaggtggac acaccaggac tttctcttcc ctgtgcagag attatttttt aaaaggtcac 
     3241 aatcaacatt cattgctgtc ggtgggttga actgtgtgga caagctcact gaacaatgaa 
     3301 tgcaactgtg gccccgcttt ttgctgtcac aatcaacaga tattccatct ttgaaagatg 
     3361 tgttcaaaat agtactattg ttctttaagt tttccaattc gtcagcatct tctggtttag 
     3421 ctagctcgag cttttggagt acgtcgtctt taggttgggg ggaggggttt tatgcgatgg 
     3481 agtttcccca cactgagtgg gtggagactg aagttaggcc agcttggcac ttgatgtaat 
     3541 tctccttgga atttgccctt tttgagtttg gatcttggtt cattctcaag cctcagacag 
     3601 tggttcaaag tttttttctt ccatttcagg tgtcgtgaaa actacccctc tagagtcgag 
     3661 ctaccggtcg ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag 
     3721 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag 
     3781 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag 
     3841 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag 
     3901 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc 
     3961 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac 
     4021 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc 
     4081 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg 
     4141 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc 
     4201 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc 
     4261 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc 
     4321 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac 
     4381 aaggacccac cggtcgccac catgaccgag tacaagccca cggtgcgcct cgccacccgc 
     4441 gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta ccccgccacg 
     4501 cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca agaactcttc 
     4561 ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg 
     4621 gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg 
     4681 cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga aggcctcctg 
     4741 gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac 
     4801 caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc 
     4861 gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt ctacgagcgg 
     4921 ctcggcttca ccgtcaccgc cgacgtcgag tgcccgaagg accgcgcgac ctggtgcatg 
     4981 acccgcaagc ccggtgcctg agcggccgcg tcgacaatca acctctggat tacaaaattt 
     5041 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 
     5101 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 
     5161 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 
     5221 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 
     5281 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 
     5341 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 
     5401 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc 
     5461 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 
     5521 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga 
     5581 tctccctttg ggccgcctcc ccgcctggaa ttcgagctcg gtacctttaa gaccaatgac 
     5641 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct 
     5701 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga 
     5761 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata 
     5821 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta 
     5881 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca 
     5941 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt 
     6001 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     6061 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 
     6121 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 
     6181 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 
     6241 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg 
     6301 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac 
     6361 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 
     6421 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 
     6481 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 
     6541 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 
     6601 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 
     6661 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 
     6721 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 
     6781 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 
     6841 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 
     6901 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg 
     6961 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 
     7021 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 
     7081 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct 
     7141 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg 
     7201 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg 
     7261 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt 
     7321 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga 
     7381 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga 
     7441 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac 
     7501 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg 
     7561 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac 
     7621 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct 
     7681 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct 
     7741 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg 
     7801 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat 
     7861 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg 
     7921 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat 
     7981 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 
     8041 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 
     8101 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 
     8161 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 
     8221 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 
     8281 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 
     8341 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 
     8401 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 
     8461 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 
     8521 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 
     8581 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 
     8641 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 
     8701 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 
     8761 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 
     8821 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 
     8881 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 
     8941 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 
     9001 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 
     9061 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 
     9121 gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgc 
//
